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Upon analysis of the absorbance of the 96-well plate (image 2), a standard curve may be developed to assess the performance of the assay via it's R-squared value (image 3)

We then expanded our search to additional databases, including the Human Metabolome Database (HMDB), the Kyoto Encyclopedia of Genes and Genomes (KEGG), and the National Center for Biotechnology Information (NCBI) Gene database

Fontana, L., Ghezzi, L., Cross, A

The results of DNA barcoding and phylogenetic analyses classify all three kratom products as Mitragyna speciosa , based on comparison with the published sequences for this species, except for the ITS analysis, which showed a conflicting outcome for sample K55

Biochimica et Biophysica Acta (BBA)-General Subjects, 2013, 1830(5): 3143-3153